Dr. Loyda Meléndez Lab
Laboratory
Dr. Wojna's laboratory facilitie consists of 300 sq. ft. It is a biosafety level-2 laboratory located
on the third floor of the Biomedical Building at the Univesity of Puerto Rico Medical Sciences Campus. These
laboratories are immediately adjacent to the other NeuroAIDS research laboratories to facilitate sharing of
equipment and optimize communications. They are in close proximity to the Clinical Research Center.
Clinical
Recruitment will be performed at primary HIV clinics after the director's support for patient
screening and evaluation. All patients' evaluations and procedures will be at the UPR MSC Clinical Research
Clinic (CRC). The CRC consists of 14,000 sq. ft space, 8 examination rooms, 3 nursing stations, 2 procedure
rooms, 2 infusion areas, 2 isolation rooms, core laboratory, record room, pharmacy, and administrative
areas. The new Informatics Core will be available for database support, web page capabilities, and video
conferences.
Computer
There are 2 Dell Intel Pentium 4 1 GHZ X processor/256 MB RAM, HP Laserjet 4100N printer.
Both are equipped with network capabilities, software for data statistical analysis, graphics, and word
processing.
Office
Dr. Wojna's office is 120 sq. ft and is located at the third floor of the Biomedical Building. There is
secretarial and administrative support. The office is equipped with telephone and internet service. Fax
machines and photocopies are available on a shared basis. The office is located in close proximity to the
laboratory.
Other
Neuropsychological Laboratory has 200 sq. ft divided into two testing rooms. It provides the latest
versions of psychological and neuropsychological tests. This laboratory is also equipped with an under optic
scan, photocopy facilities, two phone extensions, a computer with Internet access and printer, and a library.
Major Equipment
Dr. Wojna's laboratory is fully equipped with laminar flow hood, ultra-low freezer, small refrigerator/freezer,
microcentrifuge, safety cabinets, vortex, hot plate and stirring, ELISA Washer and Reader, electronic
balance, PH meter, benchtop sterilizer, autoclave, and nitrogen liquid storage facility.
Dr. Wojna's laboratory is in close proximity to other laboratories at the Neuro-AIDS SNRP, Drs. Loyda Melendez and Carlos
Luciano. Dr. Melendez has all the necessary equipment for the culture of monocytes and viruses including
HIV and Dr. Luciano has all the equipment related to the analsysis of skin nerve fibers. The main building of
the UPR MSC has common equipment rooms at the Physiology Department (6th floor of main building)
These rooms provide the use of VersaDoc System (BioRad), Beckman Ultra Centrifuges, Sartorious
microbalance, Ultra-pure water facilities and ice-maker machine. In addition, also at the Physiology
Department, there is a core facility, Microscopy Image Center, initially developed by the Research Center for
Minority Institutions and now under the supervision of the physiology faculty. This core facility consists of
Shimadzu spectrofluorometer, AMICO Bowman series 2 spectronic unicam, Zeiss Confocal Microscope,
Zeiss Axioscope Microscope, Zeiss Axiovert Microscope, Xillix Microimager Video Camera, Dage Video
Camera, Nikon Automatic Camera, Leica Cryostat, and vibratome.
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