Dr. José A. Lasalde-Dominicci Lab

Laboratory
The main occupies 1800 square feet in the Biology Building (Julio Garcia Diaz 111- 115; see www.nachrs.org). A common area of 400 Sq. ft. shared common instruments is also available.

Animal
The mice used in this research are housed in the animal facility is located in lower floor of the Department of Biology in Julio Garcia building. A 320 Sq. ft. is available in the animal care facilities to the mice lines. In total the Animal housing rooms has approximately 3,000 square feet. The housing animal is modern and provided with tables, water, stable electricity (110 and 240 V), animal yard, incinerator area, isolation room, biohazard area, treatment room, surgery and recovery room, food storage and diet room, necropsy room, and autoclave room. The animal care facility is under supervision of a full time animal technologist with two trained aides and a consultant veterinarian. This is a centralized service responsible for the care of laboratory animal, and for looking after their general health.

Computer
The laboratory has four Pentium PC based computers (one Pentium III) and two Hewlett Packard laser Jet.

Office
The PI has a about 300 square feet office adjacent to JGD 111 Other: Other supporting facilities include a staffed tissue culture facility and radioimmunoassay room. The current laboratory personnel include, eleven Ph.D. students, two postdocts, 2 technicians and eleven undergraduate students from the Biology and Chemistry departments.

Major Equipment (see www.nachrs.org for detailed information)
Electrophysiological and Molecular Biology equipment in Julio Garcia Diaz 115 includes: micropipette puller, pHmeter, osmometer, analytical balances, two patch and three voltage-clamp units with fast perfusion systems to exchange solutions, DNA sequencing gel system, FPLC, refrigerators, electrophoresis equipment, bio-rad gel documentation system, savant, incubator with orbital shaking, ultra low temperature freezer (-80 ¼C), and microcentrifuges. A Carl Zeiss 510 NLO multiphoton photon confocal imaging facility, see www.cifupr.org.

Departmental Facilities (Shared Equipment)
Includes: Scintillation counter, Fluorescent microscope, Molecular imager data collection, electron microscope, Fluor S MultiImager for scanning of double fluorescence images, gel documentation system, NMR: General Electric QE-300 and Bruker Avance DPX-300 (multinuclear) 300MHz spectrometers, and a Bruker Avanve DRX 500 MHz system (multinuclear, variable temperature, X nucleus third channel, inverse detection), FTIR: Nicolet 740 FT-IR system, GC-MS: HP 5995A, HP-5971A and high resolution VG-Fisons Autospect MS (FAB, CI, DIP) system located at our Departamental MS facility. X-Ray: Bruker P4 (Cu radiation) and Bruker SMART CCD (Mo radiation) diffractometers located at our RXD facility, Polarimeter Perking Elmer 243B, Micromass VG Quatro II mass spectrometer equipped with an electrospray ionization interface.